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2. | | DAMATTA, F. M.; RONCHI, C. P.; ARAÚJO, J. B. S. O café Conilon em sistemas agroflorestais. In: FONSECA, A. F. A. da.; SAKIYMA, N.; BORÉM, A. (Ed.). Café Conilon do plantio à colheita. Viçosa, MG : UFV, cap. 9, p. 185-203, 2015.Biblioteca(s): Biblioteca Rui Tendinha. |
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3. | | DAMATTA, F. M.; RONCHI, C. P.; SALES, E. F.; ARAÚJO, J. B. S. O café Conilon em sistemas agroflorestais. In: FERRÃO, R. G.; FONSECA, A. F. A. da.; FERRÃO, M. A. G.; DE MUNER, L. H. (Ed.). Café Conilon. 2 ed. atual. ampli. Vitória, ES: Incaper, p. 481-493, 2017.Biblioteca(s): Biblioteca Rui Tendinha. |
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4. | | DAMATTA, F. M.; ROCNHI, C. P.; SALES, E. F.; ARAÚJO, J. B. S. Conilon coffee in agroforesiry systems. In: FERRÃO, R. G.; FONSECA, A. F. A. da.; FERRÃO, M. A. G.; DE MUNER, L. H. (Ed.). Conilon Coffee. 3 edition updated and expanded Vitória, ES : Incaper, 2019. Cap. 19, p. 593-609. Translated from: Café Conilon, 2017 - Incaper. English translation: Marcele Gualda Pasolini.Biblioteca(s): Biblioteca Rui Tendinha. |
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5. | | PRAXEDES, S. C.; DAMATTA, F. M.; LOUREIRO, M. E.; FERRÃO, M. A. G.; CORDEIRO, A. T. Effects of long-term soil drought on photosynthesis and carbohydrate metabolism in mature robusta coffee (Coffea canephora Pierre var. kouillou) leaves. Environmental and Experimental Botany, v. 56, n. 3, p. 263-273, 2006,Biblioteca(s): Biblioteca Rui Tendinha. |
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6. | | RONCHI, C. P.; GUARÇONI M., A.; COMÉRIO, F.; COSTA, J. M.; VOLPI, P. S.; VERDIN FILHO, A. C.; FONSECA, A. F. A. da.; DAMATTA, F. M. Efeito de épocas de poda na produtividade de clones de café conilon de diferentes épocas de maturação dos frutos. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 33., 2007, Lavras. Anais... Brasília, DF: Embrapa Café, 2009.Biblioteca(s): Biblioteca Rui Tendinha. |
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7. | | SILVA, V. A.; ANTUNES, W. C.; GUIMARÃES, B. L. S.; PAIVA, R. M. C.; SILVA, V. de F.; FERRÃO, M. A. G.; DAMATTA, F. M.; LOUREIRO, M. E. Resposta fisiológica de clone de café Conilon sensível à deficiência hídrica enxertado em porta-enxerto tolerante. Pesquisa Agropecuária Brasileira, Brasília v.45, n.5, p.457-464, maio. 2010.Biblioteca(s): Biblioteca Rui Tendinha. |
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8. | | SILVA, V. A.; ANTUNES, W. C.; GUIMARÃES, B. L. S.; ALMEIDA, A. M.; FERRÃO, M. A. G.; DaMATTA, F. M.; LOUREIRO, M. E. Uso de porta-enxertos para aumentar a tolerância a seca em Coffea canephora Pierre. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. 5p.Biblioteca(s): Biblioteca Rui Tendinha. |
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9. | | MARRACCINI, P.; SILVA, V. A. da.; ELBELT, S.; GUIMARÃES, B. L. S.; LOUREIRO, M. E.; DAMATTA, F. M.; FERRÃO, M. A. G.; FONSECA, A. F. A. da.; SILVA, F. R. da.; ANDRADE, A. C. Análise da expressão de genes candidatos para a tolerância à seca em folhas de clones de Coffea canephora var. Conillon, caracterizados fisiologicamente. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. 5p.Biblioteca(s): Biblioteca Rui Tendinha. |
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10. | | MORAIS, L. E.; CAVATTE, P. C.; MEDINA, E. F.; SILVA, P. E. M.; MARTINS, S. C. V.; VOLPI, P. S.; ANDRADE JÚNIOR, S.; MACHADO FILHO, J. A.; RONCHI, C. P.; DAMATTA, F. M. The effects of pruning at different times on the growth, photosynthesis and yeld of conilon coffea (Coffea Canephora) clones with varyng patterns of fruit maturation in southeastern Brasil. Experimental Agriculture, 48, pp 210-221, 2012. 12 p.Biblioteca(s): Biblioteca Rui Tendinha. |
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11. | | SILVA, V. A.; PRADO, F. M.; ANTUNES, W. C.; PAIVA, R. M. C.; FERRÃO, M. A. G.; ANDRADE, A. C.; DI MASCIO, P.; LOUREIRO, M. E.; DaMATTA, F. M.; ALMEIDA, A. M. Reciprocal grafting between clones with contrasting drought tolerance suggests a key role of abscisic acid in coffee acclimation to drought stress. Plant Growth Regulation, v. 84, n. 250, p. 1-9, 2018.Biblioteca(s): Biblioteca Rui Tendinha. |
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12. | | MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012.Biblioteca(s): Biblioteca Rui Tendinha. |
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Registros recuperados : 12 | |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
13/01/2015 |
Data da última atualização: |
13/01/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
Thesagro: |
Café; Coffea canephora; Genetics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03034naa a2200445 a 4500 001 1004907 005 2015-01-13 008 2012 bl uuuu u00u1 u #d 100 1 $aMARRACCINI, P. 245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource] 260 $c2012 520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. 650 $aCafé 650 $aCoffea canephora 650 $aGenetics 653 $aCandidate gene 653 $aCoffea canephora 653 $aDifferential expression 653 $aDrought acclimation 653 $aGenética 653 $aProteomic 653 $aProteômica 653 $aReal time PCR 700 1 $aVINECKY, F. 700 1 $aALVES, G. S. C. 700 1 $aRAMOS, H. J. O. 700 1 $aELBELT, S. 700 1 $aVIEIRA, N. G. 700 1 $aCARNEIRO, F. A. 700 1 $aSUJII, P. S. 700 1 $aALEKCEVETCH, J. C. 700 1 $aSILVA, V. A. 700 1 $aDaMATTA, F. M. 700 1 $aFERRÃO, M. A. G. 700 1 $aLEROY, T. 700 1 $aPOT, D. 700 1 $aVIEIRA, L. G. E. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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